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It typically is able to make the menstrual cycle normal by estrogen and progestogen-induced downregulation of hypothalamus-pituitary axis, resulting in decrease free testosterone concentrations and lowering free circulating androgens [49]

The response to GIP is also markedly impaired in patients with T2DM

This review describes the physiological and pathophysiological mechanisms governing the actions of estradiol during the menstrual cycle on food intake and energy expenditure and how estradiol acts with other weight-controlling molecules such as GLP-1

Then, total RNA (2 g) was reversely transcribed into cDNA using Iscript cDNA Synthesis Kit (Bio-Rad, Milan, Italy), following the manufacturers instructions